Wulff J C, Deeg H J, Storb R
Transplantation. 1982 Jun;33(6):616-20. doi: 10.1097/00007890-198206000-00010.
A murine monoclonal antibody (DT-2) is described which has been raised against canine thymocytes. DT-2 activates complement and is reactive with most canine thymocytes, peripheral blood T cells, thoracic duct lymphocytes, bronchoalveolar lymphocytes, and T chronic lymphatic leukemia cells. The antibody is nonreactive with surface immunoglobulin-positive blood lymphocytes, monocytes, bronchoalveolar macrophages, null acute lymphatic leukemia cells, granulocytes, erythrocytes, and platelets. In mixed lymphocyte cultures DT-2 and complement eliminate the responding but not the stimulating cell population. Mitogen stimulation (phytohemagglutinin, concanavalin A) experiments revealed that the responding cell affected by DT-2 is of lymphoid and not of monocyte/macrophage origin. All our data suggest that DT-2 is an antibody reacting specifically with canine T cells.
描述了一种针对犬胸腺细胞产生的鼠单克隆抗体（DT-2）。DT-2可激活补体，并与大多数犬胸腺细胞、外周血T细胞、胸导管淋巴细胞、支气管肺泡淋巴细胞以及T慢性淋巴细胞白血病细胞发生反应。该抗体与表面免疫球蛋白阳性的血液淋巴细胞、单核细胞、支气管肺泡巨噬细胞、无急性淋巴细胞白血病细胞、粒细胞、红细胞和血小板无反应。在混合淋巴细胞培养中，DT-2和补体可消除反应性细胞群体，但不消除刺激性细胞群体。丝裂原刺激（植物血凝素、刀豆球蛋白A）实验表明，受DT-2影响的反应性细胞来源于淋巴细胞而非单核细胞/巨噬细胞。我们所有的数据表明，DT-2是一种与犬T细胞特异性反应的抗体。
