Wulff J C, Tsoi M S, Aprile J, Deeg H J, Durkopp N, Storb R
Blut. 1982 Nov;45(5):309-16. doi: 10.1007/BF00319524.
Canine blood lymphocytes were nonlytically separated on antibody-coated petri dishes into surface immunoglobulin-positive (SIg+) and -negative (SIg-) populations. SIg- cells were further separated into cells reactive or non-reactive with monoclonal antibody DT-2 recognizing canine T lymphocytes. The purity of the three enriched lymphocyte populations exceeded 90% as assessed by immunofluorescence. Mitogen stimulation showed a vigorous response of SIg+ cells to pokeweed mitogen and concanavalin A but only a weak response to phytohemagglutinin. In mixed DT-2- and DT-2+ cells responded to phytohemagglutinin, concanavalin A and pokeweed mitogen, and both populations were good responders in mixed leukocyte culture. Only DT-2- cells were potent stimulators; DT-2+ cells were not. Hence, canine blood T cells can be divided into two subsets, DT-2+ and DT-2-, both of which are responsive to mitogens and alloantigens.
犬血淋巴细胞在抗体包被的培养皿上被非裂解性分离为表面免疫球蛋白阳性（SIg+）和阴性（SIg-）群体。SIg-细胞进一步被分离为与识别犬T淋巴细胞的单克隆抗体DT-2反应或不反应的细胞。通过免疫荧光评估，这三种富集淋巴细胞群体的纯度超过90%。丝裂原刺激显示SIg+细胞对商陆丝裂原和刀豆球蛋白A有强烈反应，但对植物血凝素只有微弱反应。在混合培养中，DT-2-和DT-2+细胞对植物血凝素、刀豆球蛋白A和商陆丝裂原均有反应，且在混合白细胞培养中这两个群体都是良好的反应者。只有DT-2-细胞是有效的刺激物；DT-2+细胞不是。因此，犬血T细胞可分为两个亚群，DT-2+和DT-2-，两者都对丝裂原和同种异体抗原有反应。
