Hinz E, Diesfeld H J, Gehrig H, Kirsten C
Tropenmed Parasitol. 1981 Dec;32(4):247-9.
Sera from 30 E. multilocularis infected NMRI-mice and 30 D. viteae infected golden hamsters were tested in the indirect fluorescent antibody (IFAT) and indirect hemagglutination (IHA) tests. In the IFAT there were cross-reactions with all sera, but there were differences between the average titres. While filarial antisera gave higher titres in the homologous system (1:640 against 1:80), it was the opposite with Echinococcus antisera (1:160 against 1:640). Other differences referred to the parasite structures which fluoresced: using Echinococcus antigen the homologous sera gave a bright fluorescence of the outer protoscolex layers including the hooks, while Dipetalonema antisera showed fluorescence of the protoscolex parenchyma only. Thus it is easy to distinguish between homologous and heterologous reactions. Corresponding differences could not be found when using Dipetalonema antigen. In the IHA tests with E. granulosus antigen, which showed an average titre of 1:640 witch E. multilocularis sera, there were few cross-reactions, only 4 out of 30 Dipetalonema antisera giving a positive result.
对30只感染多房棘球绦虫的NMRI小鼠和30只感染魏氏棘唇线虫的金黄仓鼠的血清进行了间接荧光抗体试验（IFAT）和间接血凝试验（IHA）。在IFAT中，所有血清均出现交叉反应，但平均滴度存在差异。丝虫抗血清在同源系统中的滴度较高（1:640对比1:80），而棘球绦虫抗血清则相反（1:160对比1:640）。其他差异涉及发出荧光的寄生虫结构：使用棘球绦虫抗原时，同源血清使包括钩在内的原头节外层发出明亮荧光，而棘唇线虫抗血清仅使原头节实质发出荧光。因此，很容易区分同源和异源反应。使用棘唇线虫抗原时未发现相应差异。在用细粒棘球绦虫抗原进行的IHA试验中，多房棘球绦虫血清的平均滴度为1:640，交叉反应较少，30份棘唇线虫抗血清中只有4份呈阳性结果。
