Bezrukova A G, Vladimirskaia I K
Tsitologiia. 1982 May;24(5):507-21.
Different methods of light scattering measurements for cell suspension and cells in flow are reviewed. The intensity of light scattered at different angles suggests information about the cell size, the nucleus to cell diameter ratio, the thickness of surface membrane, the changes in the internal cell structure state, and about the heterogeneity of cell population. The efficiency of information obtained from the turbidity measurements depends on the geometry of the spectrophotometer. Measurements made with diaphragms allow us to get information about the mean size of biological particles, their form, refractive index, concentration etc. The main advantages of light scattering methods are their high sensitivity, a possibility to use the same species before and after the experiment, and a compatibility with other methods.
本文综述了用于细胞悬浮液和流动细胞的不同光散射测量方法。在不同角度散射的光强度可提供有关细胞大小、细胞核与细胞直径比、表面膜厚度、细胞内部结构状态变化以及细胞群体异质性的信息。从浊度测量中获得信息的效率取决于分光光度计的几何结构。使用光阑进行测量可使我们获得有关生物颗粒的平均大小、形状、折射率、浓度等信息。光散射方法的主要优点是灵敏度高、实验前后可使用同一物种以及与其他方法具有兼容性。
