纤溶酶抑制剂相互作用。α2-巨球蛋白存在时α2-纤溶酶抑制剂的有效性。
Plasmin inhibitor interactions. The effectiveness of alpha2-plasmin inhibitor in the presence of alpha2-macroglobulin.
作者信息
Harpel P C
出版信息
J Exp Med. 1977 Oct 1;146(4):1033-40. doi: 10.1084/jem.146.4.1033.
Abstract
alpha2-Plasmin inhibitor and alpha2-macroglobulin were allowed to compete for the protease plasmin. The binding of the enzyme to these inhibitors was assessed by two different but comparable methods. The interactions were completed in 10 s of incubation, and transfer of plasmin from one inhibitor to the other did not occur. Almost as much plasmin was bound to alpha2-plasmin inhibitor in mixtures containing a large molar excess of alpha2-macroglobulin relative to plasmin or alpha2-plasmin inhibitor, as was bound in mixtures not containing alpha2-macroblobulin. These studies demonstrate directly the effectiveness of alpha2-plasmin inhibitor in binding and inhibiting plasmin in the presence of alpha2-macroglobulin, and suggest that the alpha2-plasmin inhibitor may be the major circulating plasmin inhibitor.
摘要
α2-纤溶酶抑制剂和α2-巨球蛋白被允许竞争蛋白酶纤溶酶。通过两种不同但可比的方法评估该酶与这些抑制剂的结合。相互作用在孵育10秒内完成，纤溶酶不会从一种抑制剂转移到另一种抑制剂。在相对于纤溶酶或α2-纤溶酶抑制剂含有大量摩尔过量α2-巨球蛋白的混合物中，与不含α2-巨球蛋白的混合物中结合的纤溶酶几乎一样多。这些研究直接证明了α2-纤溶酶抑制剂在存在α2-巨球蛋白的情况下结合和抑制纤溶酶的有效性，并表明α2-纤溶酶抑制剂可能是主要的循环纤溶酶抑制剂。
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