Nakib N M, Bissada N F, Simmelink J W, Goldstine S N
J Periodontol. 1982 Jun;53(6):368-78. doi: 10.1902/jop.1982.53.6.368.
This study was undertaken to determine the extent of in vitro penetration of E. coli endotoxin into the root cementum of periodontally healthy and diseased teeth. Freshly extracted teeth were washed in distilled water, scaled and divided into two groups of 16 teeth each. Nine diseased and five healthy teeth in the first group were immersed in various concentrations of E. coli endotoxin for 2 to 12 weeks. They were then prepared for indirect immunofluorescence examination after treatment with anti-endotoxin antibody and rhodamine conjugated secondary antibody. Teeth in the second group were prepared for autoradiographic examination by immersing nine diseased and five healthy teeth in tritium labelled E. coli endotoxin for 2 to 12 weeks. The latter technique also allowed for semi-quantitative study of the depth of endotoxin penetration by creating facets on the root at various depths after endotoxin exposure. This technique was also used to investigate the strength of endotoxin binding to the tooth surface by brushing for 1 minute and re-examining the tooth for the presence of endotoxin. Controls included periodontally diseased and healthy teeth. Results of the study showed that (1) endotoxin adheres to the tooth surface without penetration into the root cementum of either periodontally healthy or diseased teeth, and (2) the binding of the endotoxin to the root surface appears to be weak.
本研究旨在确定大肠杆菌内毒素在体外对牙周健康和患病牙齿的牙根牙骨质的渗透程度。新鲜拔除的牙齿用蒸馏水冲洗、刮治，然后分为两组，每组16颗牙齿。第一组中9颗患病牙齿和5颗健康牙齿浸泡在不同浓度的大肠杆菌内毒素中2至12周。在用抗内毒素抗体和罗丹明偶联二抗处理后，对其进行间接免疫荧光检查。第二组中的牙齿通过将9颗患病牙齿和5颗健康牙齿浸泡在氚标记的大肠杆菌内毒素中2至12周来进行放射自显影检查。后一种技术还可以通过在内毒素暴露后在牙根不同深度处制作小平面来对内毒素渗透深度进行半定量研究。该技术还用于通过刷牙1分钟并重新检查牙齿内毒素的存在来研究内毒素与牙齿表面结合的强度。对照组包括牙周病牙齿和健康牙齿。研究结果表明：（1）内毒素附着于牙齿表面，未渗透到牙周健康或患病牙齿的牙根牙骨质中；（2）内毒素与牙根表面的结合似乎较弱。
