Spaander P J, Ruiter D J, Hermans J, de Voogt H J, Brussee J A, Boon M E
Anal Quant Cytol. 1982 Jun;4(2):123-7.
A pilot study of selective morphometry was performed in smear preparations from fine needle aspirates of six cytologically graded prostatic carcinomas. Given the instruction to select carcinoma cells with large nuclei for measurement, cytotechnologists and a cytopathologist differed in the selection of cells from grade III tumors. The cytotechnologists had discarded large bare atypical nuclei because they assumed that these were degenerated cells. Transmission electron microscopy, using an open-face embedding technique, demonstrated, however, that these cells were not degenerated but were poorly differentiated carcinoma cells that had lost their cytoplasm during smear preparation. It is obviously of great importance for the morphometric discrimination of grade I and grade III carcinomas that such bare atypical nuclei be included in the selections of cells to be measured. Our data indicate that the measurement of 20 selected cells per smear preparation is sufficient for accurate morphometric grading and that such measurements are reproducible.
对6例经细胞学分级的前列腺癌细针穿刺涂片标本进行了选择性形态测量的初步研究。在接到选择大核癌细胞进行测量的指令后，细胞技术人员和一位细胞病理学家在III级肿瘤细胞的选择上存在差异。细胞技术人员丢弃了大的裸核非典型细胞，因为他们认为这些是退化细胞。然而，采用开放式包埋技术的透射电子显微镜显示，这些细胞并非退化细胞，而是在涂片制备过程中失去细胞质的低分化癌细胞。对于I级和III级癌的形态测量鉴别而言，将此类裸核非典型细胞纳入待测量细胞的选择显然极为重要。我们的数据表明，每份涂片制备测量20个选定细胞足以进行准确的形态测量分级，且此类测量具有可重复性。
