David H L, Rastogi N, Frehel C, Gheorghiu M
Ann Microbiol (Paris). 1982 Jul-Aug;133(1):129-39.
The purpose of this investigation was to examine those properties of Mycobacterium leprae which might be useful in estimating the heterogeneity of the bacterial populations harvested from the tissues of experimentally infected armadillos. The following technical procedures were applied: fluorescent microscopy on smears stained by an Auramine O-Ethidium bromide dual procedure, fine structure observation of ultrathin sections, reduction of potassium tellurite as observed under the electron microscope, and ATP content of the bacteria. The quantitative data from these procedures was compared to the morphological index, and it was shown that the results did not correlate. However, the study of tellurite reduction was interesting because this technique may prove useful in evaluating the contamination of M. leprae preparations by host tissues. More significant was the fact that M. leprae reduced tellurite. Even though the reduction was at a very low efficiency under the conditions described, it would be possible to use this technique to investigate the effects of several substrates on the respiratory activity of the leprosy bacilli.
本研究的目的是检测麻风分枝杆菌的那些特性，这些特性可能有助于评估从实验感染犰狳组织中收获的细菌群体的异质性。采用了以下技术程序：用金胺O-溴化乙锭双重染色法对涂片进行荧光显微镜检查、超薄切片的精细结构观察、在电子显微镜下观察亚碲酸钾的还原以及细菌的ATP含量。将这些程序的定量数据与形态学指标进行比较，结果表明两者不相关。然而，亚碲酸盐还原研究很有趣，因为该技术可能被证明有助于评估麻风分枝杆菌制剂被宿主组织污染的情况。更重要的是麻风分枝杆菌能还原亚碲酸盐这一事实。尽管在所描述的条件下还原效率非常低，但利用该技术研究几种底物对麻风杆菌呼吸活性的影响是可行的。
