Franzblau S G, Hastings R C
Antimicrob Agents Chemother. 1987 May;31(5):780-3. doi: 10.1128/AAC.31.5.780.
Measurement of intracellular ATP of Mycobacterium leprae after direct in vitro exposure to antimicrobial agents was evaluated as a rapid means of identifying potentially useful therapeutic agents. Nude mouse-derived M. leprae was incubated in an axenic modified Dubos medium in the presence or absence of antimicrobial agents for up to 3 weeks. ATP was then assayed by using the firefly bioluminescence technique. Rifampin, clofazimine, and ethionamide each effected a significantly accelerated rate of ATP decay compared with controls. Dapsone appeared inactive, possibly reflecting a general insensitivity of this system to compounds acting at certain loci. The system appeared suitable for assessing comparative activity of new structural analogs of clofazimine. Other active compounds included erythromycin, minocycline, chloramphenicol, gramicidin, and, to a lesser extent, cycloserine, cephalothin, ciprofloxacin, tetracycline, and gramicidin S. The penicillins, bacitracin, isoniazid, nalidixic acid, trimethoprim, polymyxin B, and griseofulvin were all inactive. The system appears sensitive to agents with various modes of action and may prove useful as a primary screen for antileprosy drugs.
将体外直接暴露于抗菌剂后的麻风分枝杆菌细胞内ATP水平测定作为一种快速识别潜在有用治疗药物的方法进行了评估。将裸鼠来源的麻风分枝杆菌在无菌改良杜氏培养基中，于有无抗菌剂存在的情况下培养长达3周。然后使用萤火虫生物发光技术测定ATP。与对照组相比，利福平、氯法齐明和乙硫异烟胺各自均使ATP衰减速率显著加快。氨苯砜似乎无活性，这可能反映了该系统对作用于某些位点的化合物普遍不敏感。该系统似乎适用于评估氯法齐明新结构类似物的比较活性。其他活性化合物包括红霉素、米诺环素、氯霉素、短杆菌肽，以及程度较轻的环丝氨酸、头孢噻吩、环丙沙星、四环素和短杆菌肽S。青霉素、杆菌肽、异烟肼、萘啶酸、甲氧苄啶、多粘菌素B和灰黄霉素均无活性。该系统似乎对具有各种作用方式的药物敏感，可能作为抗麻风病药物的初步筛选方法而有用。
