Topping D L, Mayes P A
Biochem J. 1982 May 15;204(2):433-9. doi: 10.1042/bj2040433.
Livers from fed rats were perfused with whole rat blood and infused with oleate to maintain constant concentrations of serum non-esterified fatty acids over a wide physiological range. Infusion of insulin opposed the antilipogenic effects of increasing concentrations of serum non-esterified fatty acids. Secretion of very-low-density-lipoprotein triacylglycerols was directly proportional to the concentration of serum non-esterified fatty acids and was increased by insulin. The secretion of newly-synthesized fatty acids in very-low-density-lipoprotein triacylglycerols decreased with increasing concentrations of serum non-esterified fatty acid. Insulin opposed this change. Cholesterol biosynthesis was unaffected by alterations in concentration of serum non-esterified fatty acid but was increased by insulin. Equilibrium concentrations of perfusate lactate and glucose were increased by serum non-esterified fatty acids but steady-state rates of hepatic glucose output and lactate uptake were unchanged. Insulin decreased perfusate glucose concentrations and abolished the increase in its concentration that resulted from increases in non-esterified fatty acid concentrations.
用全血灌注喂食大鼠的肝脏，并注入油酸，以在较宽的生理范围内维持血清非酯化脂肪酸浓度恒定。注入胰岛素可对抗血清非酯化脂肪酸浓度升高的抗脂肪生成作用。极低密度脂蛋白三酰甘油的分泌与血清非酯化脂肪酸浓度成正比，并因胰岛素而增加。极低密度脂蛋白三酰甘油中新合成脂肪酸的分泌随血清非酯化脂肪酸浓度升高而减少。胰岛素可对抗这种变化。胆固醇生物合成不受血清非酯化脂肪酸浓度变化的影响，但因胰岛素而增加。灌注液中乳酸和葡萄糖的平衡浓度因血清非酯化脂肪酸而升高，但肝脏葡萄糖输出和乳酸摄取的稳态速率未变。胰岛素降低了灌注液葡萄糖浓度，并消除了因非酯化脂肪酸浓度升高导致的葡萄糖浓度升高。
