Denissen H W, de Groot K, Makkes P C, van den Hooff A, Klopper P J
J Biomed Mater Res. 1980 Nov;14(6):713-21. doi: 10.1002/jbm.820140603.
Dense (97-99.9%) hydroxylapatite ceramics were implanted in muscle and bone tissue of rats. The aim of this study was to compare apatite implant material made of commercially available powder with laboratory powder prepared in a painstaking manner. Biocompatibility, biostability, and adherence to bone were evaluated. In muscle tissue the implants were found to be encapsulated with a very thin connective tissue layer. Implantation in excavated bone tissue resulted with new bone directly deposited against the implant surface, irrespective of the type of hydroxylapatite used. When the implants were protruding from the bone surface, bone appeared to grow up to the edge of the protruding part of the implant. A very strong bonding developed; push-out tests indicated that the bone fractured but never at the interface. Histologic studies proved that a sleeve of newly formed bone closely encased the implant, regardless of shape. It was concluded that dense apatite ceramics are fully compatible with the tibia of the rat and that no degradation of the implant material occurred for intervals of up to 6 months after implantation. The very strong bonding without mechanical retention indicated continuity between artificial hydroxylapatite and natural bone. No difference was found between the biological behavior of the hydroxylapatites prepared from commercial or laboratory starting powders.
将致密（97 - 99.9%）的羟基磷灰石陶瓷植入大鼠的肌肉和骨组织中。本研究的目的是比较由市售粉末制成的磷灰石植入材料与精心制备的实验室粉末。对生物相容性、生物稳定性和与骨的附着性进行了评估。在肌肉组织中，发现植入物被一层非常薄的结缔组织层包裹。植入挖掘出的骨组织后，无论使用何种类型的羟基磷灰石，新骨都直接沉积在植入物表面。当植入物从骨表面突出时，骨似乎生长到植入物突出部分的边缘。形成了非常强的结合；推出试验表明骨会断裂，但从未在界面处断裂。组织学研究证明，无论形状如何，新形成的骨套紧密包裹着植入物。得出的结论是，致密的磷灰石陶瓷与大鼠胫骨完全相容，植入后长达6个月的时间内植入材料没有发生降解。没有机械固定的情况下非常强的结合表明人工羟基磷灰石与天然骨之间具有连续性。由商业或实验室起始粉末制备的羟基磷灰石的生物学行为之间没有发现差异。
