Ellies L G, Carter J M, Natiella J R, Featherstone J D, Nelson D G
Eastman Dental Center, Department of Oral Biology, Rochester, New York 14620.
J Biomed Mater Res. 1988 Feb;22(2):137-48. doi: 10.1002/jbm.820220206.
Studies have shown synthetic calcium phosphates such as hydroxyapatite and beta tricalcium phosphate to be biocompatible in vivo. However, few studies have quantitated histological responses to the implants. The aim of this study was to develop a method for the quantitative assessment of tissue biocompatibility to ceramic materials in vivo and to use this method to compare noncarbonated and carbonated apatite implants. Synthetic sintered apatites of 0, 3, and 6% carbonate by weight were prepared and cut into implants 4 X 4 X 1 mm. These were placed 2 mm into the medial aspect of rat femurs. Following sacrifice at 4 weeks, the femurs were fixed in formalin, demineralized in formic acid, and embedded in glycol methacrylate. Sections were cut on an ultramicrotome set at 1.5 micron and stained with toluidine blue. A point counting technique using standard stereological grids and a low-power microscope was used to measure areas of new bone formation. The width of the connective tissue zone adjacent to muscle was measured using an image analyzer. All implants were well accepted by the host tissues judging from criteria of minimal inflammation and degree of fixation. Results showed an increase in new bone formed in the marrow cavity with increasing carbonate content. This may improve stability of the implant in the host bone, particularly during the initial healing period. A technique which should enable quantitative histological evaluation of different ceramic materials has been developed. The use of this method indicates that further studies are warranted to investigate carbonated apatite as an implant material.
研究表明，合成磷酸钙，如羟基磷灰石和β-磷酸三钙，在体内具有生物相容性。然而，很少有研究对植入物的组织学反应进行定量分析。本研究的目的是开发一种在体内定量评估组织对陶瓷材料生物相容性的方法，并使用该方法比较非碳酸化和碳酸化磷灰石植入物。制备了重量比为0%、3%和6%的合成烧结磷灰石，并将其切割成4×4×1毫米的植入物。将这些植入物置于大鼠股骨内侧2毫米处。4周后处死大鼠，将股骨固定在福尔马林中，用甲酸脱钙，然后包埋在乙二醇甲基丙烯酸酯中。使用超薄切片机切成1.5微米厚的切片，并用甲苯胺蓝染色。使用标准的体视学网格和低倍显微镜的点计数技术来测量新骨形成的面积。使用图像分析仪测量与肌肉相邻的结缔组织区的宽度。从最小炎症和固定程度的标准判断，所有植入物均被宿主组织良好接受。结果表明，随着碳酸盐含量的增加，骨髓腔内形成的新骨增加。这可能会提高植入物在宿主骨中的稳定性，尤其是在初始愈合期。已经开发出一种能够对不同陶瓷材料进行定量组织学评估的技术。该方法的应用表明，有必要进一步研究碳酸化磷灰石作为植入材料。
