Stevenson D K, Hsu Y P, McMorrow M, Berseth C L, Neu J, Miller J J
Eur J Pediatr. 1980 Sep;134(3):255-9. doi: 10.1007/BF00441482.
Determinations of C3, C4, and C5 concentrations by radial immunodiffusion, and assays for the activation products of C3, C3c and C3d by counterimmunoelectrophoresis, were performed on 80 infants. Seven nonbacteremic preterm infants with necrotizing enterocolitis (NEC) or probable NEC (PNEC) were found at the time of diagnosis to have a significantly lower mean concentration of C3 (P less than 0.05, 1-tailed) without C3 activation when compared to other noninfected preterm infants. Ten full-term and 63 preterm infants were studied prospectively during the first days of life, and were then followed for the postnatal development of localized or systemic infection. Assays for the detection of C3 activation products were negative in all these infants. Four preterm infants who developed PNEC after 5 or more days without clinical illness had low original concentrations of complement components. The pathogenesis of NEC may not involve primarily complement activation, and susceptibility to this condition may be related to pre-existing deficiencies in complement component concentrations relative to gestational age, or to defective activation of C3 in the presence of certain bacterial species and strains.
采用放射免疫扩散法测定了80例婴儿的C3、C4和C5浓度，并通过对流免疫电泳法检测了C3的激活产物C3c和C3d。诊断时发现7例患有坏死性小肠结肠炎（NEC）或可能患有NEC（PNEC）的非菌血症早产儿，与其他未感染的早产儿相比，其C3平均浓度显著降低（P<0.05，单尾），且无C3激活。对10例足月儿和63例早产儿在出生后的头几天进行了前瞻性研究，随后对其出生后局部或全身感染的发展情况进行了随访。所有这些婴儿检测C3激活产物的结果均为阴性。4例在5天或更长时间内无临床疾病后发生PNEC的早产儿，其补体成分的初始浓度较低。NEC的发病机制可能主要不涉及补体激活，对这种疾病的易感性可能与相对于胎龄预先存在的补体成分浓度缺陷有关，或者与在某些细菌种类和菌株存在的情况下C3激活缺陷有关。
