Lewensohn R, Ringborg U, Hansson J
Cancer Res. 1982 Jan;42(1):84-8.
Unscheduled DNA synthesis (UDS) indicated by melphalan was studied in freshly collected tumor cells from human melanoma metastases. Comparative studies were done on human bone marrow blast cells. Significant levels of UDS comparable with those in myeloblasts were found in only two of eight melanoma cell populations. This difference between melanoma and blast cells was not related to different cellular uptake of melphalan. When UDS was induced by ultraviolet irradiation, significant levels of UDS were found in all melanoma and blast cell populations studied. Also, in a human melanoma cell line, high levels of UDS were found after exposure to ultraviolet irradiation, while treatment with melphalan did not result in detectable levels of UDS. Possible explanations for the divergent results of UDS in melphalan-exposed melanoma cells are discussed.
在从人类黑色素瘤转移灶新鲜采集的肿瘤细胞中，研究了美法仑所指示的非程序性DNA合成（UDS）。对人类骨髓原始细胞进行了比较研究。在八个黑色素瘤细胞群体中，只有两个群体发现了与成髓细胞相当的显著水平的UDS。黑色素瘤细胞与原始细胞之间的这种差异与美法仑的不同细胞摄取无关。当通过紫外线照射诱导UDS时，在所有研究的黑色素瘤和原始细胞群体中都发现了显著水平的UDS。此外，在一种人类黑色素瘤细胞系中，紫外线照射后发现了高水平的UDS，而用美法仑处理则未导致可检测到的UDS水平。讨论了在暴露于美法仑的黑色素瘤细胞中UDS结果不同的可能解释。
