Jaynes P K, Feld R D, Johnson G F
Clin Chem. 1982 Jan;28(1):114-7.
We describe a procedure for specific, rapid, kinetic determination of creatinine, in which a manual coupled-enzyme micro-scale assay is adapted to a centrifugal analyzer. The creatinine reaction is ultimately linked to NADH utilization, which is measured by the absorbance change at 340 nm. This procedure requires 15 microL of serum and the standard curve is linear to a creatinine concentration of 200 mg/L. A four-point kinetic algorithm allows the dynamic range of the assay to be extended without sacrificing sensitivity, and makes a separate serum blank unnecessary. The within-run precision (CV) for samples with a creatinine concentration of 11 and 52 mg/L was 5.6 and 2.4%, respectively; day-to-day CV for a creatinine concentration of 11 mg/L was 7.7% (n = 21). We compared this procedure with a kinetic Jaffé procedure, with excellent agreement (r = 0.996; y = 0.96x + 2.4 mg/L). Bilirubin, non-esterified fatty acids, and ketone bodies do not affect creatinine determinations by this method; thus the method is especially useful for monitoring the renal function of diabetics.
我们描述了一种用于特异性、快速、动力学测定肌酐的方法，其中手动偶联酶微量测定法适用于离心分析仪。肌酐反应最终与NADH的利用相关，通过340nm处吸光度的变化来测量。该方法需要15μL血清，标准曲线在肌酐浓度为200mg/L时呈线性。四点动力学算法可在不牺牲灵敏度的情况下扩展测定的动态范围，并且无需单独的血清空白。肌酐浓度为11和52mg/L的样本批内精密度（CV）分别为5.6%和2.4%；肌酐浓度为11mg/L的样本日间CV为7.7%（n = 21）。我们将该方法与动力学Jaffé法进行了比较，结果具有良好的一致性（r = 0.996；y = 0.96x + 2.4mg/L）。胆红素、非酯化脂肪酸和酮体不影响该方法测定肌酐；因此该方法对于监测糖尿病患者的肾功能特别有用。
