Margrey M, Margrey K, Bruns D E, Boyd J C, Fortier G A, Renoe B W, Savory J
Ann Clin Lab Sci. 1984 Jul-Aug;14(4):298-303.
A method is described for enzymatic measurement of serum creatinine using a centrifugal analyzer. The method employs fixed-time kinetics and a true serum blank. The method required 100 microliters of serum and is linear to 90 mg per L. Day-to-day precision studies revealed a standard deviation (coefficient of variation) of less than +/- 0.5 mg per L (7.4 percent) at a mean creatinine concentration of 6 mg per L and +/- 0.5 mg per L (1.8 percent) at a mean value of 28 mg per L. The method was free of interferences from acetoacetic acid, ascorbic acid, bilirubin, and hemoglobin. The speed, precision, and accuracy of the method suggest that it represents a useful alternative to methods using the alkaline picrate procedure.
描述了一种使用离心分析仪酶法测定血清肌酐的方法。该方法采用固定时间动力学和真正的血清空白。该方法需要100微升血清，线性范围至90mg/L。日常精密度研究显示，在肌酐平均浓度为6mg/L时，标准差（变异系数）小于±0.5mg/L（7.4%）；在平均值为28mg/L时，标准差为±0.5mg/L（1.8%）。该方法不受乙酰乙酸、抗坏血酸、胆红素和血红蛋白的干扰。该方法的速度、精密度和准确性表明，它是使用碱性苦味酸盐法的方法的一种有用替代方法。
