Brown L M, Duck-Chong C G, Hensley W J
Clin Chem. 1982 Feb;28(2):344-8.
Many laboratories have found that their procedure for determining lecithin/sphingomyelin (L/S)ratios gives an unacceptably high proportion of false predictions of fetal lung immaturity. We investigated each step in the procedure, in an attempt to improve the clinical performance of the test and to make the method more amenable to standardization between laboratories. L/S ratios were determined by the new procedure in amniotic fluid from 147 pregnancies, collected within two days of delivery of the infant. Four cases had an L/S ratio less than 2; all developed hyaline membrane disease. No other cases of hyaline membrane disease were encountered in this study. For 106 of these pregnancies, the L/S ratio was also determined by the procedure previously in use. This predicted lung immaturity for 16 infants, only four of whom developed hyaline membrane disease. All 12 cases incorrectly predicted as immature by the old procedure were correctly classified by the new procedure.
许多实验室发现，他们测定卵磷脂/鞘磷脂（L/S）比值的方法对胎儿肺不成熟的预测存在比例高得令人无法接受的错误结果。我们对该方法的每一步骤进行了研究，试图提高检测的临床性能，并使该方法更易于在各实验室之间实现标准化。采用新方法对147例妊娠孕妇分娩前两日采集的羊水进行L/S比值测定。4例L/S比值小于2，均发生了透明膜病。本研究未发现其他透明膜病病例。其中106例妊娠孕妇的L/S比值也采用之前使用的方法进行了测定。该方法预测16例婴儿肺不成熟，其中仅4例发生了透明膜病。旧方法错误预测为不成熟的12例病例，新方法均正确分类。
