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导入K562细胞中的人类珠蛋白基因的差异表达。

Differential expression of human globin genes introduced in K562 cells.

作者信息

Kioussis D, Wilson F, Khazaie K, Grosveld F

出版信息

EMBO J. 1985 Apr;4(4):927-31. doi: 10.1002/j.1460-2075.1985.tb03720.x.

DOI:10.1002/j.1460-2075.1985.tb03720.x
PMID:2990914
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554281/
Abstract

We have constructed a series of hybrid globin genes between the 5' half of the human epsilon-, gamma- and beta-globin genes, and the 3' half of the rabbit beta-globin gene. These hybrid genes were introduced into the cell line K562. Analysis of the hybrid mRNA populations show that only the hybrid epsilon- and gamma-globin genes are expressed in these cells in concordance with the endogenous K562 globin gene expression. It is therefore likely that these cells contain transcriptional factors which allow the specific expression of introduced epsilon- and gamma-, but not beta-globin genes, irrespective of their chromosomal localization.

摘要

我们构建了一系列杂种珠蛋白基因，这些基因由人类ε-、γ-和β-珠蛋白基因的5'端一半与兔β-珠蛋白基因的3'端一半组成。这些杂种基因被导入细胞系K562。对杂种mRNA群体的分析表明，在这些细胞中，只有杂种ε-和γ-珠蛋白基因与内源性K562珠蛋白基因的表达一致地表达。因此，这些细胞很可能含有转录因子，这些转录因子允许导入的ε-和γ-珠蛋白基因而非β-珠蛋白基因进行特异性表达，而不论它们的染色体定位如何。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/36331ca93803/emboj00269-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/c79cadf9c527/emboj00269-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/35d0affb425f/emboj00269-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/36331ca93803/emboj00269-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/c79cadf9c527/emboj00269-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/35d0affb425f/emboj00269-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f88/554281/36331ca93803/emboj00269-0085-a.jpg

相似文献

1
Differential expression of human globin genes introduced in K562 cells.导入K562细胞中的人类珠蛋白基因的差异表达。
EMBO J. 1985 Apr;4(4):927-31. doi: 10.1002/j.1460-2075.1985.tb03720.x.
2
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Stable transfer and expression of exogenous human globin genes in human erythroleukemia (K562) cells.外源人珠蛋白基因在人红白血病（K562）细胞中的稳定转移与表达。
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Transcriptional regulation of globin gene expression in the human erythroid cell line K562.人红系细胞系K562中珠蛋白基因表达的转录调控。
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6
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Accurate initiation of human epsilon-globin RNA synthesis by Escherichia coli RNA polymerase in isolated nuclei of K562 erythroleukemia cells.大肠杆菌RNA聚合酶在K562红白血病细胞分离细胞核中准确起始人ε-珠蛋白RNA合成。
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A beta-globin gene, inactive in the K562 leukemic cell, functions normally in a heterologous expression system.一种在K562白血病细胞中无活性的β-珠蛋白基因，在异源表达系统中正常发挥功能。
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Inducible transcription of five globin genes in K562 human leukemia cells.K562人白血病细胞中五个珠蛋白基因的可诱导转录。
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引用本文的文献

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Developmental regulation of the human embryonic beta-like globin gene is mediated by synergistic interactions among multiple tissue- and stage-specific elements.人类胚胎β样珠蛋白基因的发育调控是由多种组织和阶段特异性元件之间的协同相互作用介导的。
Mol Cell Biol. 1993 Dec;13(12):7457-68. doi: 10.1128/mcb.13.12.7457-7468.1993.
3
Stable gene transfer and tissue-specific expression of a human globin gene using adenoviral vectors.

本文引用的文献

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Hemoglobin expression in clones of K562 cell line.K562细胞系克隆中的血红蛋白表达。
Eur J Biochem. 1982 Jan;121(3):649-55. doi: 10.1111/j.1432-1033.1982.tb05835.x.
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Differential expression of the globin genes in human leukemia K562(S) cells induced to differentiate by hemin or butyric acid.人白血病K562(S)细胞经氯化血红素或丁酸诱导分化后珠蛋白基因的差异表达
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EMBO J. 1986 Sep;5(9):2377-85. doi: 10.1002/j.1460-2075.1986.tb04507.x.
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Activation of the human beta-globin promoter in K562 cells by DNA sequences 5' to the fetal gamma- or embryonic zeta-globin genes.胎儿γ-珠蛋白基因或胚胎ζ-珠蛋白基因5'端的DNA序列对K562细胞中人β-珠蛋白启动子的激活作用。
J Clin Invest. 1987 Aug;80(2):374-80. doi: 10.1172/JCI113082.
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Human globin gene promoter sequences are sufficient for specific expression of a hybrid gene transfected into tissue culture cells.人类珠蛋白基因启动子序列足以使转染到组织培养细胞中的杂交基因实现特异性表达。
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Differential expression of mouse beta/goat beta c, mouse beta/goat beta F, and mouse beta/goat epsilon II hybrid globin genes in murine erythroleukemia cells.小鼠β/山羊βc、小鼠β/山羊βF和小鼠β/山羊εII杂交珠蛋白基因在鼠红细胞白血病细胞中的差异表达。
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9
The -117 mutation in Greek HPFH affects the binding of three nuclear factors to the CCAAT region of the gamma-globin gene.希腊遗传性胎儿血红蛋白持续增多症（HPFH）中的 -117 突变影响三种核因子与γ-珠蛋白基因 CCAAT 区域的结合。
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10
Promoter sequences required for function of the human gamma globin gene in erythroid cells.人类γ珠蛋白基因在红细胞中发挥功能所需的启动子序列。
EMBO J. 1986 Jan;5(1):121-6. doi: 10.1002/j.1460-2075.1986.tb04185.x.
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6
Regulated expression of the human beta-globin gene family in murine erythroleukaemia cells.人β-珠蛋白基因家族在小鼠红白血病细胞中的调控表达。
Nature. 1983;305(5932):333-6. doi: 10.1038/305333a0.
7
Transcriptional regulation of globin gene expression in the human erythroid cell line K562.人红系细胞系K562中珠蛋白基因表达的转录调控。
Science. 1983 Jun 17;220(4603):1281-3. doi: 10.1126/science.6574602.
8
The regulated expression of beta-globin genes introduced into mouse erythroleukemia cells.导入小鼠红白血病细胞中的β-珠蛋白基因的调控表达。
Cell. 1983 Feb;32(2):483-93. doi: 10.1016/0092-8674(83)90468-3.
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Transfer of human globin genes to erythroleukemic mouse cells.将人类珠蛋白基因转移至红白血病小鼠细胞。
EMBO J. 1982;1(1):15-20. doi: 10.1002/j.1460-2075.1982.tb01117.x.
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Inducible transcription of five globin genes in K562 human leukemia cells.K562人白血病细胞中五个珠蛋白基因的可诱导转录。
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5515-9. doi: 10.1073/pnas.80.18.5515.