Smith G N
Immunology. 1982 Jan;45(1):163-7.
Cyclosporin A (CSA) has been used in an attempt to suppress the primary immune response of HgA(A)-negative rabbits to A-positive red cells. The immune response was assessed by measuring the survival of a small intravenous (i.v.) dose of 51Cr-labelled A-positive cells and by testing the serum of the immunized rabbits for anti-A. In one experiment, eight A-negative rabbits were given a first i.v. injection of A-positive red cells, and CSA (25 mg/kg/day) in olive oil was given by mouth for 17-34 days. There was no evidence of impaired alloimmunization compared with the responses in control animals treated with olive oil alone. In a second experiment, eight A-negative rabbits were given a first injection of A-positive muscularly (i.m.), and CSA (25 mg/kg/day) in miglyol was given by im.m. injection for 10 days. Six of these rabbits were rendered unresponsive, and the remaining two, who showed impaired survival of the monitoring red cells, produced only low anit-A titres. Seven out of eight controls given i.m. miglyol without CSA responded with good anti-A production. Rabbits that were unresponsive to A-positive red cells responded normally to sheep red blood cells 15 weeks after CSA treatment. Higher serum levels of CSA were found following i.m. administration of the drug but treatment by this route as associated with severe toxicity in some rabbits.
环孢素A（CSA）已被用于尝试抑制HgA(A)阴性兔对A阳性红细胞的初次免疫反应。通过测量小剂量静脉注射（i.v.）51Cr标记的A阳性细胞的存活情况以及检测免疫兔血清中的抗A抗体来评估免疫反应。在一项实验中，给8只A阴性兔首次静脉注射A阳性红细胞，并口服橄榄油中的CSA（25毫克/千克/天），持续17至34天。与仅用橄榄油处理的对照动物的反应相比，没有证据表明同种免疫受到损害。在第二项实验中，给8只A阴性兔首次肌肉注射（i.m.）A阳性细胞，并通过肌肉注射给予Miglyol中的CSA（25毫克/千克/天），持续10天。其中6只兔无反应，其余2只监测红细胞存活受损的兔仅产生低抗A滴度。8只接受肌肉注射Miglyol但未使用CSA的对照兔中有7只产生了良好的抗A反应。对A阳性红细胞无反应的兔在CSA治疗15周后对绵羊红细胞反应正常。肌肉注射该药物后发现血清中CSA水平较高，但这种给药途径与一些兔的严重毒性有关。
