Smith G N, Mollison P L
Immunology. 1974 May;26(5):865-75.
Successive injections of Hg-positive red cells were given at intervals of 2–3 months to Hg-negative rabbits. In forty-four out of fifty-one animals, red cell survival became progressively shorter and, in the majority of instances, anti-Hg was found in the serum. Characteristically, the first survival curve was initially normal but showed `collapse' after a mean interval of 17 days; following subsequent injections, cells were cleared at an accelerated rate. In the remaining seven Hg-negative rabbits the survival of Hg-positive red cells was either repeatedly normal or was subnormal after the first one or two injections and became almost normal following further injections. In four out of six of these animals, following additional injections of Hg-positive cells given i.m. with FCA, anti-Hg became detectable in the serum. Thus, some animals which appeared to be non-responders to Hg as judged by normal survival of Hg-positive red cells after i.v. injection alone showed some response when antigen was also given i.m. with adjuvant.
每隔2至3个月给Hg阴性的兔子连续注射Hg阳性红细胞。在51只动物中，有44只动物的红细胞存活时间逐渐缩短，并且在大多数情况下，血清中发现了抗Hg。其特征是，第一条存活曲线最初正常，但在平均17天后出现“崩溃”；随后注射后，细胞清除速度加快。在其余7只Hg阴性兔子中，Hg阳性红细胞的存活要么反复正常，要么在第一次或第二次注射后低于正常水平，而在进一步注射后几乎恢复正常。在其中6只动物中的4只中，肌肉注射Hg阳性细胞并加弗氏完全佐剂（FCA）后，血清中可检测到抗Hg。因此，一些仅通过静脉注射后Hg阳性红细胞正常存活而看似对Hg无反应的动物，当抗原也与佐剂一起肌肉注射时会表现出一些反应。
