使用二乙氨基乙基硅胶柱和薄层色谱法分离磷脂。
Separation of phospholipids using a diethylaminoethyl-silica gel column and thin-layer chromatography.
作者信息
Do U H, Lo S L
出版信息
J Chromatogr. 1986 Sep 5;381(2):233-40. doi: 10.1016/s0378-4347(00)83589-6.
Phospholipids derived from egg yolk are readily separated by DEAE-silica gel column chromatography using stepwise gradient elution. The overall recovery of phospholipids from the column is 85-95% at a loading capacity of 120 mg of lipids per 10 g of DEAE-silica gel. The complete separation of eight phospholipids (phosphatidylcholine, sphingomyelin, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine and lysophosphatidylserine; 5 micrograms each) is also achieved by one-dimensional DEAE-silica gel thin-layer chromatography with the solvent system chloroform-methanol-water-pyridine-58% ammonia solution (130:55:8:4:4, v/v).
源自蛋黄的磷脂可通过使用梯度洗脱的DEAE硅胶柱色谱轻松分离。在每10克DEAE硅胶的负载量为120毫克脂质的情况下，从柱中回收磷脂的总体回收率为85 - 95%。使用氯仿 - 甲醇 - 水 - 吡啶 - 58%氨水溶液（130:55:8:4:4，v/v）的溶剂系统，通过一维DEAE硅胶薄层色谱法也能实现8种磷脂（磷脂酰胆碱、鞘磷脂、溶血磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰丝氨酸和溶血磷脂酰丝氨酸；各5微克）的完全分离。
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