Bounameaux H, Duckert F, Walter M, Bounameaux Y
Thromb Haemost. 1978 Jun 30;39(3):607-15.
At III levels measured with six methods in 36 people: 10 healthy controls, 10 women taking a progestagen Lynestrenol and 16 women taking a combined oestrogen-progestagen contraceptive pill. The reproducibility and the sensitivity of these methods as well as the correlation between methods were studied. The Hensen and Loeliger technique had a poor reproducibility and the results obtained with the Howie technique were not in good correlation with those obtained with other methods, especially in the group taking oral contraceptives. The two amidolytic methods using chromogenic substrates were found to be very accurate for antithrombin III activity determination. The rocket immunoelectrophoresis was more accurate and more practical than the radial immunodiffusion. With all methods--except the one of Howie--the 10 women taking progestagen Lynestrenol and the 16 taking combined oestrogen-progestagen had a low AT III and differed significantly from the 10 normals tested. No difference was observed between women taking progestagen only or an oestrogen-progestagen combination.
对36人采用六种方法进行Ⅲ级检测：10名健康对照者、10名服用炔诺酮的女性以及16名服用复方雌激素 - 孕激素避孕药的女性。研究了这些方法的重现性、敏感性以及方法之间的相关性。亨森（Hensen）和勒利格（Loeliger）技术的重现性较差，豪伊（Howie）技术所得结果与其他方法所得结果相关性不佳，尤其是在服用口服避孕药的人群中。发现两种使用显色底物的酰胺水解法在抗凝血酶Ⅲ活性测定方面非常准确。火箭免疫电泳比单向免疫扩散更准确、更实用。除豪伊方法外，所有方法检测结果显示，10名服用炔诺酮的女性和16名服用复方雌激素 - 孕激素的女性抗凝血酶Ⅲ水平较低，与10名正常受试者有显著差异。仅服用孕激素或服用雌激素 - 孕激素复方制剂的女性之间未观察到差异。
