Stavridis I C, Vorias N I
Thromb Haemost. 1978 Jun 30;39(3):631-9.
A simple, quantitative method for detection of small amounts of heparin in human plasma is described. This method is based on the activation of coagulation factor X to its enzymatic form (Xa), by a mixture of RVV platelet substitute (Esnouf and Williams 1962, Esnouf and Jobin 1967) in a plasma rendered fibrinogen free without affecting other clotting factores (Bell et al. 1968, Bell 1973), by Arvin (Ancrod). Activation of factor X, results in thrombin generation. Thrombin inactivation by its natural inhibitors depends on the time elapsed from the start-point of activation. The inactivation process is enhanced by heparin (Biggs et al. 1970, Blombäck et al. 1963) and this enhancement depends on the quantity of heparin in the plasma.
本文描述了一种检测人血浆中少量肝素的简单定量方法。该方法基于在不含纤维蛋白原的血浆中，通过RVV血小板替代物（埃斯努夫和威廉姆斯，1962年；埃斯努夫和若宾，1967年）的混合物将凝血因子X激活为其酶形式（Xa），且不影响其他凝血因子（贝尔等人，1968年；贝尔，1973年），阿文（安克洛）也有相关作用。因子X的激活会导致凝血酶生成。凝血酶被其天然抑制剂灭活取决于从激活起点开始经过的时间。肝素会增强灭活过程（比格斯等人，1970年；布洛姆贝克等人，1963年），且这种增强作用取决于血浆中肝素的量。
