Pullen A H
J Neurosci Methods. 1982 Jan;5(1-2):103-20. doi: 10.1016/0165-0270(82)90057-7.
The BASIC program developed for use on a M.S.I. 6800 microprocessor analyses cross-sectional measurements of fibre (D) and axon (d) calibre made on electron micrographs of suitable magnification. Data input routines cover measurements of area and diameter, made manually or presented as fully computed values printed out by a M.O.P. Separate sub-routines input digitized data from a digitizer tablet coupled to the microprocessor. Myelin sheath thickness (2M) (D-d), or area and 'g' (d/D) are derived. The two analytical programs provide either frequency distributions, with associated statistics for each parameter, or analyses of the relationships (D:d), (d:2M) and (D:g). The highly structured nature of the program permits it to be edited or extended according to the project. The main programme controls the overall flow of analysis; discrete subprograms inserted into the main program perform the data input, parameter derivations, data checks and analyses. This system offers maximum flexibility and is suitable for most morphometric investigations involving peripheral nerves.
为在中规模集成6800微处理器上使用而开发的BASIC程序，可分析在适当放大倍数的电子显微镜照片上所做的纤维（D）和轴突（d）管径的横断面测量。数据输入程序涵盖手动测量的面积和直径，或由微操作处理器打印输出的完全计算值。单独的子程序输入来自与微处理器相连的数字化仪平板的数字化数据。可得出髓鞘厚度（2M）（D - d）、面积和“g”（d/D）。这两个分析程序可提供每个参数的频率分布及相关统计数据，或分析（D:d）、（d:2M）和（D:g）之间的关系。该程序高度结构化，允许根据项目进行编辑或扩展。主程序控制分析的整体流程；插入主程序的离散子程序执行数据输入、参数推导、数据检查和分析。该系统具有最大的灵活性，适用于大多数涉及周围神经的形态测量研究。
