Roda A, Roda E, Festi D, Aldini R, Mazzella G, Sama C, Barbara L
Steroids. 1978 Jul-Aug;32(1):13-24. doi: 10.1016/0039-128x(78)90094-6.
A sensitive and specific radioimmunoassay for glycine and taurine conjugates of lithocholic acid (CLCA) has been developed. 3H-glycolithocholic acid (S.A. = 17Ci/mmol) was used as tracer. Separation of free from antibody-bound bile acid was carried out using ammonium sulphate (saturated solution). The antiserum showed high specificity for both glyco and tauro conjugated lithocholate (100% cross reaction) and lithocholic acid (25% cross reaction). The sensitivity of the assay (1 pmole/tube), was adequate for measuring CLCA in peripheral blood and hepatic tissue in man.
已开发出一种用于检测石胆酸甘氨酸和牛磺酸缀合物（CLCA）的灵敏且特异的放射免疫分析法。使用3H-甘氨石胆酸（比活度 = 17Ci/mmol）作为示踪剂。采用硫酸铵（饱和溶液）进行游离胆汁酸与抗体结合胆汁酸的分离。该抗血清对甘氨和牛磺缀合石胆酸盐（交叉反应率100%）以及石胆酸（交叉反应率25%）均显示出高特异性。该分析方法的灵敏度（1皮摩尔/管）足以测定人体外周血和肝组织中的CLCA。
