Cowen A E, Korman M G, Hofmann A F, Turcotte J, Carter J A
J Lipid Res. 1977 Nov;18(6):692-7.
A simple, rapid, precise radioimmunoassay for total unsulfate species of lithocholate (lithocholyglycine, lithocholyltaurine, and lithocholate) in serum is described. Antiserum was raised in rabbits by injection of lithocholylglycine coupled to bovine serum albumin (prepared by a carbodiimide method) and emulsified in complete Freund's adjuvant; antisera capable of measuring 40-120 pmol at 1:400 dilution were obtained. The tracer was [11, 12-3[H]lithocholyglycine. The radioimmunoassay featured a 2-hr binding step at 42 decrease C and a 1-hr separation step using polyethylene glycol. The antibody had the following relative specificities: lithocholyglycine and lithocholytaurine, 1; lithocholate, 1.5; chenodeoxycholyglycine, 20; and deoxycholylglycine, 55. There was no binding of various other free or conjugated sulfated and unsulfated bile acids. The mean fasting-state level in 50 healthy subjects was 0.3 mu mol/l (0.14 microgram/ml), but 11 of the 50 subjects had level too low to measure by this technique.
本文描述了一种用于测定血清中石胆酸（石胆酰甘氨酸、石胆酰牛磺酸和石胆酸）总非硫酸化形式的简单、快速、精确的放射免疫分析法。通过注射与牛血清白蛋白偶联的石胆酰甘氨酸（采用碳二亚胺法制备）并在完全弗氏佐剂中乳化，在兔体内产生抗血清；获得了在1:400稀释度下能够检测40 - 120 pmol的抗血清。示踪剂为[11, 12 - 3[H]石胆酰甘氨酸。该放射免疫分析法的特点是在42℃下进行2小时的结合步骤，并使用聚乙二醇进行1小时的分离步骤。该抗体具有以下相对特异性：石胆酰甘氨酸和石胆酰牛磺酸为1；石胆酸为1.5；鹅去氧胆酰甘氨酸为20；脱氧胆酰甘氨酸为55。各种其他游离或结合的硫酸化和非硫酸化胆汁酸均无结合。50名健康受试者的空腹状态平均水平为0.3 μmol/l（0.14 μg/ml），但50名受试者中有11人的水平过低，无法用该技术测量。
