Reyer R W
Am J Anat. 1982 Jan;163(1):25-45. doi: 10.1002/aja.1001630103.
Early stages in lens regeneration from the dorsal margin of the pupil, following removal of the young lens from the embryonic eyecup, were studied with transmission electron microscopy in Notophthalmus viridescens. At the stage of operation, the eyecup cells have an undifferentiated, embryonic appearance with numerous free ribosomes and scattered mitochondria. In normal embryonic eyes containing a lens, the iris epithelium differentiates from the edge of the optic cup by growth and flattening of the cells to form a two-layered cuboidal epithelium. There is extensive melanogenesis in both inner and outer layers. In lentectomized eyes, this flattening does not occur and melanogenesis takes place mainly in the outer layer of epithelium. Mitoses are abundant and a vesicle of unpigmented columnar cells, enclosed by a basal lamina, develops from the dorsal pupillary margin of the eyecup. Macrophages are present and phagocytize some of the melanin but less than during dedifferentiation of pigmented iris epithelium. During this regeneration of a lens vesicle, there is an increase in the number of polyribosomes, granular endoplasmic reticulum, and mitochondria in preparation for synthesis of the lens proteins. Micropinocytosis occurs at the cells surfaces.
利用透射电子显微镜对绿红东美螈（Notophthalmus viridescens）胚胎眼杯中的幼晶状体摘除后，从瞳孔背缘进行晶状体再生的早期阶段进行了研究。在手术阶段，眼杯细胞呈现未分化的胚胎外观，有大量游离核糖体和散在的线粒体。在含有晶状体的正常胚胎眼中，虹膜上皮通过细胞生长和扁平从视杯边缘分化形成两层立方上皮。内外层均有广泛的黑色素生成。在晶状体摘除的眼中，这种扁平现象不发生，黑色素生成主要发生在上皮外层。有丝分裂丰富，一个由基膜包围的无色素柱状细胞囊泡从眼杯的背侧瞳孔边缘发育而来。存在巨噬细胞，其吞噬一些黑色素，但比色素虹膜上皮去分化期间吞噬的要少。在晶状体囊泡再生过程中，多核糖体、粗面内质网和线粒体数量增加，为晶状体蛋白的合成做准备。细胞表面发生微胞饮作用。
