Del Rio-Tsonis K, Tomarev S I, Tsonis P A
Department of Biology, University of Dayton, Ohio 45469-2320, USA.
Invest Ophthalmol Vis Sci. 1999 Aug;40(9):2039-45.
To determine the expression pattern of Prox 1 during the process of lens regeneration in the urodele Notophthalmus viridescens.
Polymerase chain reaction was performed to amplify a partial newt Prox 1 sequence. In situ hybridization and immunodetection methods were used to detect the Prox 1 mRNA and the Prox 1 protein, respectively.
Prox 1 mRNA was present in the retina and in the lens (in the epithelium and bow region) of the intact eye. Prox 1 protein was found to be predominantly present in the lens and dorsal iris of the intact eye, although some trace levels of Prox 1 protein were detected in the ventral iris as well. After lentectomy, expression of the mRNA was also pronounced in the dorsal dedifferentiating iris and the regenerating lens. The ventral iris also expressed Prox 1 but seemingly at lower levels. Although Prox 1 protein showed upregulation in the dorsal iris during the process of lens regeneration, trace levels were also detected in the ventral iris. In the retina, Prox 1 protein was distributed in horizontal cells of the inner nuclear layer, whereas the mRNA was expressed in all layers of the retina.
Prox 1 was unevenly distributed in the intact cells of the newt iris, with significantly higher levels of Prox 1 protein present in the dorsal versus the ventral margin. This protein was differentially regulated during the process of lens regeneration, with obvious upregulation in the dorsal iris. Prox 1 is the first transcriptional factor to be shown to be regulated in the dorsal versus ventral iris during the process of lens regeneration.
确定Prox 1在有尾目动物绿红东美螈晶状体再生过程中的表达模式。
采用聚合酶链反应扩增部分蝾螈Prox 1序列。分别使用原位杂交和免疫检测方法检测Prox 1 mRNA和Prox 1蛋白。
Prox 1 mRNA存在于完整眼睛的视网膜和晶状体（上皮和弓状区域）中。发现Prox 1蛋白主要存在于完整眼睛的晶状体和背侧虹膜中，尽管在腹侧虹膜中也检测到了一些微量的Prox 1蛋白。晶状体切除术后，mRNA在背侧去分化虹膜和再生晶状体中也有明显表达。腹侧虹膜也表达Prox 1，但水平似乎较低。虽然在晶状体再生过程中Prox 1蛋白在背侧虹膜中上调，但在腹侧虹膜中也检测到微量表达。在视网膜中，Prox 1蛋白分布在内核层的水平细胞中，而mRNA在视网膜的所有层中均有表达。
Prox 1在蝾螈虹膜的完整细胞中分布不均，背侧边缘的Prox 1蛋白水平明显高于腹侧。该蛋白在晶状体再生过程中受到差异调节，在背侧虹膜中明显上调。Prox 1是第一个被证明在晶状体再生过程中在背侧和腹侧虹膜中受到调节的转录因子。
