Thitoff Angela R, Call Mindy K, Del Rio-Tsonis Katia, Tsonis Panagiotis A
Laboratory of Molecular Biology, Department of Biology, University of Dayton, Dayton, Ohio 45469-2320, USA.
Anat Rec A Discov Mol Cell Evol Biol. 2003 Mar;271(1):185-8. doi: 10.1002/ar.a.10023.
Based on the role of retinoblastoma (Rb) in lens development and in the cell cycle reentry of muscle cells during limb regeneration, we have analyzed expression or Rb patterns in intact and lens regeneration-undergoing newt eyes. We find that in intact newt eye Rb is expressed in the retina as a gradient with higher levels in the photoreceptor layer and virtually no expression in the ganglion layer. In addition, a second gradient was detected within the photoreceptor layer with expression diminishing at the dorsal and ventral regions. In the intact lens, Rb is expressed in the lens epithelium and in the differentiating lens fibers at the bow region. During lens regeneration, Rb is expressed very strongly in the differentiating lens fibers, but not in the lens epithelium. Using an antibody specific to the hyperphosphorylated form of Rb, we detected the inactive protein only in the pigment epithelial cells of the iris. These distinct patterns might be related to the regenerative potential of the lens in the newt.
基于视网膜母细胞瘤（Rb）在晶状体发育以及肢体再生过程中肌肉细胞细胞周期重新进入方面的作用，我们分析了完整的和正在进行晶状体再生的蝾螈眼睛中Rb的表达或模式。我们发现，在完整的蝾螈眼睛中，Rb在视网膜中呈梯度表达，在光感受器层水平较高，而在神经节层几乎不表达。此外，在光感受器层内检测到第二个梯度，其表达在背侧和腹侧区域减少。在完整的晶状体中，Rb在晶状体上皮和弓状区域正在分化的晶状体纤维中表达。在晶状体再生过程中，Rb在正在分化的晶状体纤维中强烈表达，但在晶状体上皮中不表达。使用针对Rb高磷酸化形式的特异性抗体，我们仅在虹膜的色素上皮细胞中检测到无活性蛋白。这些不同的模式可能与蝾螈晶状体的再生潜力有关。
