Wolinsky J S, Jubelt B, Burke S, Narayan O
Ann Neurol. 1982 Jan;11(1):59-68. doi: 10.1002/ana.410110111.
To determine the origin of the inflammatory response, and in particular the microglial rod cell response, in acute viral encephalitis, 4-week-old Swiss mice were injected with tritiated thymidine to label actively dividing cells prior to infection with the Lansing type 2 strain of poliovirus. As expected, the majority of polymorphonuclear and mononuclear leukocytes within the central nervous system perivascular infiltrates were shown to be hematogenous in origin. As early as 24 hours after infection, isotope-labeled cells having light histological and ultrastructural features consistent with microglia and microglial rod cells were identified within brain parenchyma and were shown to participate in neuronophagia and formation of glial nodules. Supraependymal and suprachoroidal cells were also shown to contain the label. However, neither endothelial cells nor pericytes contained label as determined by electron microscopy. These studies support a hematogenous origin for all cellular elements of the classic inflammatory response in viral infections of brain.
为了确定急性病毒性脑炎中炎症反应的起源，特别是小胶质棒状细胞反应的起源，在4周龄的瑞士小鼠感染兰辛2型脊髓灰质炎病毒之前，注射氚标记的胸腺嘧啶核苷以标记活跃分裂的细胞。正如预期的那样，中枢神经系统血管周围浸润中的大多数多形核白细胞和单核白细胞显示为血源性。早在感染后24小时，在脑实质内就鉴定出具有与小胶质细胞和小胶质棒状细胞一致的轻度组织学和超微结构特征的同位素标记细胞，并显示它们参与噬神经细胞作用和胶质结节的形成。室管膜上和脉络膜上细胞也显示含有标记物。然而，通过电子显微镜确定，内皮细胞和周细胞均不含标记物。这些研究支持了脑病毒感染中经典炎症反应的所有细胞成分均起源于血源性的观点。
