Jemionek J F, MacVittie T J, Byrne P J, Schein P S, Walden D A
Br J Haematol. 1982 Feb;50(2):257-67. doi: 10.1111/j.1365-2141.1982.tb01916.x.
Bone marrow cells from murine, canine, primate and human donors were fractionated by counterflow centrifugation-elutriation (CCE) using a continuous albumin gradient. Fractionation of 9 x 10(8) nucleated bone marrow cells (without prior removal of the erythrocytes) can be done in less than 1.5 h with 92% nucleated cell recovery, depending on donor species. Murine bone marrow cell recovery after fractionation averaged 52% whereas cell recoveries for canine, primate and human donors averaged 85%, 92% and 89%, respectively. The fractions were evaluated for total nucleated cell counts and granulocyte-macrophage colony-forming units (GM-CFC). Each species studied presented a unique profile for nucleated cell recovery and associated GM-CFC activity. These profile variations may suggest significant differences in the modes of GM-CFC expression in the individual species that may depend on cell-to-cell interactions. Although CCE is unable to isolate a single population responsible for GM-CFC activity, CCE does permit a rapid, reproducible fractionation of a large numbers of cells with minimal manipulation of sample. This makes the isolated sample ideal for further purification of progenitor cell populations
使用连续白蛋白梯度通过逆流离心淘析法（CCE）对来自小鼠、犬、灵长类和人类供体的骨髓细胞进行分级分离。根据供体物种的不同，9×10⁸个有核骨髓细胞（无需预先去除红细胞）的分级分离可在不到1.5小时内完成，有核细胞回收率达92%。分级分离后，小鼠骨髓细胞回收率平均为52%，而犬、灵长类和人类供体的细胞回收率分别平均为85%、92%和89%。对各分级部分进行有核细胞总数和粒细胞-巨噬细胞集落形成单位（GM-CFC）评估。所研究的每个物种在有核细胞回收率和相关GM-CFC活性方面都呈现出独特的特征。这些特征差异可能表明各个物种中GM-CFC表达模式存在显著差异，这可能取决于细胞间相互作用。尽管CCE无法分离出负责GM-CFC活性的单一细胞群体，但CCE确实能够在对样品进行最少操作的情况下，对大量细胞进行快速、可重复的分级分离。这使得分离出的样品成为进一步纯化祖细胞群体的理想材料。
