Lane J, Brown M, Bernstein I, Wilcox P K, Slichter S J, Nowinski R C
Br J Haematol. 1982 Feb;50(2):351-9. doi: 10.1111/j.1365-2141.1982.tb01926.x.
A solid phase platelet antibody assay has been developed which rapidly and sensitively detects PlA1 antibodies. The three-step assay is performed by: (1) adhering platelets to the wheels of a microtitre plate, (2) incubating the platelets with test serum, and (3) adding radiolabelled Staphylococcal protein A which binds to the Fc domain of IgG antibodies. Immune reactions are detected by overnight autoradiography. Characterization of the PlA1 antigen was performed by using PlA1 antisera in immune precipitation assays. A 90 000 dalton molecular weight species was precipitated from PlA1 positive human and dog platelets.
已开发出一种固相血小板抗体检测方法，可快速、灵敏地检测血小板血型抗原1（PlA1）抗体。该三步检测法的操作步骤如下：（1）将血小板粘附于微量滴定板的孔板上；（2）用待测血清孵育血小板；（3）加入与IgG抗体Fc结构域结合的放射性标记葡萄球菌蛋白A。通过过夜放射自显影检测免疫反应。采用PlA1抗血清在免疫沉淀试验中对PlA1抗原进行鉴定。从PlA1阳性的人和犬血小板中沉淀出一种分子量为90000道尔顿的物质。
