Quirk A V, Pain R H, Davison M, Shuster S
Cell Tissue Kinet. 1982 Jan;15(1):31-8. doi: 10.1111/j.1365-2184.1982.tb01021.x.
An attempt has been made to purify factors present in aqueous extracts of pig epidermis which inhibit epidermal cell proliferation. A lipophilic factor of low molecular weight (less than 10,000), has been shown to inhibit DNA synthesis as measured by the uptake of tritiated thymidine in mouse ear epidermis. Purification by alcohol precipitation, ethyl acetate extraction and silicic acid column chromatography produced a fifteen-fold increase in the specific activity of the inhibitory action. It seems likely that aggregation or absorption of this low molecular weight factor may explain the high molecular weight of epidermal cell proliferation inhibitors previously studied, as well as the difficulty in their characterization.
人们已尝试纯化猪表皮水提物中存在的抑制表皮细胞增殖的因子。一种低分子量（小于10,000）的亲脂性因子已被证明可抑制DNA合成，这是通过测量氚标记胸腺嘧啶在小鼠耳表皮中的摄取来确定的。通过乙醇沉淀、乙酸乙酯萃取和硅酸柱色谱法进行纯化，使抑制作用的比活性提高了15倍。这种低分子量因子的聚集或吸附似乎可以解释先前研究的表皮细胞增殖抑制剂的高分子量以及对其进行表征的困难。
