豆科根瘤菌中色氨酸基因的调控
Regulation of tryptophan genes in Rhizobium leguminosarum.
作者信息
Holmgren E, Crawford I P
出版信息
J Bacteriol. 1982 Mar;149(3):1135-7. doi: 10.1128/jb.149.3.1135-1137.1982.
Abstract
Twelve tryptophan auxotrophs of Rhizobium leguminosarum were characterized biochemically. They were grown in complex and minimal media with several carbon sources, in both limiting and excess tryptophan. Missing enzyme activities allowed assignment of all mutant to the trpE, trpD, trpB, or trpA gene, confirming earlier results with the same mutants (Johnston et al., Mol. Gen. Genet. 165:323-330, 1978). In regulatory experiments, only the first enzyme of the pathway, anthranilate synthase, responded (about 15-fold) to tryptophan excess or limitation.
摘要
对12株豌豆根瘤菌色氨酸营养缺陷型菌株进行了生化特性分析。它们在含有多种碳源的复合培养基和基本培养基中培养，色氨酸浓度分别处于限制水平和过量水平。通过缺失的酶活性，可将所有突变体定位到trpE、trpD、trpB或trpA基因，这证实了对相同突变体的早期研究结果（约翰斯顿等人，《分子与普通遗传学》165:323 - 330，1978年）。在调控实验中，该途径的首个酶——邻氨基苯甲酸合酶，仅对色氨酸过量或受限有反应（约15倍）。
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