Stoolman L M, Rosen S D
J Cell Biol. 1983 Mar;96(3):722-9. doi: 10.1083/jcb.96.3.722.
We are investigating the hypothesis that carbohydrate-binding molecules on the cell surface are involved in the recirculation of lymphocytes from the bloodstream into lymphoid organs. This phenomenon requires the specific attachment of circulating lymphocytes to the endothelial cells of postcapillary venules. Using an in vitro assay to measure the adhesive interaction between lymphocytes and postcapillary venules, we have found that L-fucose, D mannose, and the L-fucose-rich, sulfated polysaccharide fucoidin specifically inhibit this binding interaction. L-fucose shows stereo-selective inhibitory activity at concentrations greater than 18 mM while fucoidin produces 50% inhibition at approximately 1-5 X 10(-8) M. Fucoidin appears to interact with the lymphocyte, and not the postcapillary venule, to inhibit binding. These data suggest that cell surface carbohydrates (fucoselike) and carbohydrate-binding molecules (cell surface lectins) may contribute to the specific attachment of lymphocytes to postcapillary venules.
我们正在研究一种假说，即细胞表面的碳水化合物结合分子参与淋巴细胞从血液再循环至淋巴器官的过程。这一现象需要循环淋巴细胞与毛细血管后微静脉的内皮细胞特异性附着。通过体外试验来测量淋巴细胞与毛细血管后微静脉之间的黏附相互作用，我们发现L-岩藻糖、D-甘露糖以及富含L-岩藻糖的硫酸化多糖岩藻依聚糖可特异性抑制这种结合相互作用。当浓度大于18 mM时，L-岩藻糖表现出立体选择性抑制活性，而岩藻依聚糖在约1 - 5×10⁻⁸ M时产生50%的抑制作用。岩藻依聚糖似乎是与淋巴细胞而非毛细血管后微静脉相互作用来抑制结合。这些数据表明，细胞表面碳水化合物（类岩藻糖）和碳水化合物结合分子（细胞表面凝集素）可能有助于淋巴细胞与毛细血管后微静脉的特异性附着。
