Pang H, Ihara M, Kuchino Y, Nishimura S, Gupta R, Woese C R, McCloskey J A
J Biol Chem. 1982 Apr 10;257(7):3589-92.
The structure of a modified nucleoside isolated from Halococcus morrhuae tRNA has been established as 1-methylpseudouridine, based on mass spectrometry and gas chromatography carried out on 0.2 A260 unit of material. Not previously found in nucleic acids, 1-methylpseudouridine appears to be unique to archaebacterial tRNA, where it replaces ribosylthymine in the T psi C loop of most of the tRNAs examined thus far.
基于对0.2个A260单位物质进行的质谱分析和气相色谱分析,从盐球菌属海藻tRNA中分离出的一种修饰核苷的结构已确定为1-甲基假尿苷。1-甲基假尿苷以前在核酸中未被发现,它似乎是古细菌tRNA所特有的,在迄今为止检测的大多数tRNA的TψC环中,它取代了核糖胸腺嘧啶。