采用液相色谱-电化学检测法测定γ-氨基丁酸。
Determination of gamma-aminobutyric acid by liquid chromatography with electrochemical detection.
作者信息
Caudill W L, Houck G P, Wightman R M
出版信息
J Chromatogr. 1982 Feb 12;227(2):331-9. doi: 10.1016/s0378-4347(00)80387-4.
PMID:7061649
Abstract
gamma-Aminobutyric acid (GABA) has been determined in rat brain by derivatization with 2,4,6-trinitrobenzenesulfonic acid. The derivative and an internal standard, 2,4,6-trinitrophenyl-delta-aminovaleric acid, are extracted into toluene and separated by reversed-phase chromatography. Electrochemical reduction of these derivatives permits picomole measurements of GABA in microgram amounts of brain tissue.
摘要
已通过用2,4,6-三硝基苯磺酸衍生化法测定大鼠脑中的γ-氨基丁酸（GABA）。衍生物和内标2,4,6-三硝基苯基-δ-氨基戊酸被萃取到甲苯中，并通过反相色谱法进行分离。这些衍生物的电化学还原使得能够对微克量脑组织中的GABA进行皮摩尔级测量。
Zotero 插件