Krstulović A M, Bertani-Dziedzic L, Bautista-Cerqueira S, Gitlow S E
J Chromatogr. 1982 Feb 12;227(2):379-89.
We describe a direct analysis for the simultaneous quantitative determination of 4-hydroxy-3-methoxyphenylacetic (homovanillic) acid and other monoamine metabolites in human lumbar cerebrospinal fluid, utilizing reversed-phase high-performance liquid chromatography with amperometric detection. In addition, a rapid isocratic separation was developed for homovanillic acid in the presence of other endogenous compounds. Twenty-five unselected diagnostic specimens of human lumbar cerebrospinal fluid were extracted with ethyl acetate and subsequently analyzed using the described method. Chromatographic peaks were identified on the basis of retention behavior and ratio of responses at several oxidation potentials. Although our quantitative results correlate well with the literature values, the data were not interpreted clinically since samples were obtained from routine, diagnostic testing of patients admitted to the medical or neurologic services at the Mount Sinai Hospital.
我们描述了一种利用反相高效液相色谱-安培检测法，同时定量测定人腰椎脑脊液中4-羟基-3-甲氧基苯乙酸（高香草酸）及其他单胺代谢物的直接分析方法。此外，还开发了一种在其他内源性化合物存在下对高香草酸进行快速等度分离的方法。用乙酸乙酯萃取25份未经挑选的人腰椎脑脊液诊断标本，随后使用所述方法进行分析。根据保留行为和几个氧化电位下的响应比来鉴定色谱峰。虽然我们的定量结果与文献值相关性良好，但由于样本取自西奈山医院内科或神经科收治患者的常规诊断检测，因此未进行临床解读。
