芳香族羟基化作为体内羟基自由基形成的一种潜在测量方法。人体体液中水杨酸酯羟基化衍生物的鉴定。
Aromatic hydroxylation as a potential measure of hydroxyl-radical formation in vivo. Identification of hydroxylated derivatives of salicylate in human body fluids.
作者信息
Grootveld M, Halliwell B
出版信息
Biochem J. 1986 Jul 15;237(2):499-504. doi: 10.1042/bj2370499.
Abstract
Attack by .OH radicals, generated by a Fenton system, upon salicylate produces 2,3-dihydroxybenzoate and 2,5-dihydroxybenzoate as major products and catechol as a minor product. H.p.l.c. separation combined with electrochemical detection was used to identify and quantify 2,3-dihydroxybenzoate and 2,5-dihydroxybenzoate in human plasma and synovial fluid. We propose that conversion of salicylate into 2,3-dihydroxybenzoate, or of other aromatic compounds into specific hydroxylated products, may be a useful assay for .OH formation in the human body.
摘要
由芬顿体系产生的·OH自由基对水杨酸盐的攻击会产生2,3-二羟基苯甲酸酯和2,5-二羟基苯甲酸酯作为主要产物，以及儿茶酚作为次要产物。采用高效液相色谱分离结合电化学检测来鉴定和定量人血浆和滑液中的2,3-二羟基苯甲酸酯和2,5-二羟基苯甲酸酯。我们提出，将水杨酸盐转化为2,3-二羟基苯甲酸酯，或将其他芳香族化合物转化为特定的羟基化产物，可能是一种用于检测人体内·OH形成的有用方法。
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