Thunold S, Boyd R, Schauenstein K, Wick G
J Histochem Cytochem. 1982 Mar;30(3):201-6. doi: 10.1177/30.3.7061822.
Frozen sections of chicken lymphoid organs were examined for lymphocyte surface antigens by antisera to T and B lymphocytes (ATS;ABS), and for the presence of immunoglobulin (Ig) G Fc and complement receptors (FcR;CR) by hemadsorption with sheep erythrocytes (E) coated with chicken IgG(EA), and E coated with rabbit IgG and chicken complement (EAC). In the spleen FcR positive cells were confined to the periellipsoidal sheaths and the germinal centers. CR positive cells were found in the same spleen areas, as well as in the medulla of bursal follicles. These lymphoid areas reacted strongly with ABS, but they also stained with neutral alpha-naphthyl butyrate esterase, and phagocytosed carbon particles were found in the periellipsoidal sheaths. Furthermore, in vivo treatment with cyclophosphamide, which resulted in pronounced B-lymphocyte depletion, did not affect FcR activity, but reduced CR activity significantly. These data indicate that the FcR activity demonstrated in tissue sections is mainly confined to mononuclear phagocytes, while the CR positive cells are mainly B lymphocytes.
利用抗T淋巴细胞和B淋巴细胞抗血清（ATS；ABS）检测鸡淋巴器官冰冻切片中的淋巴细胞表面抗原，并用包被有鸡IgG的绵羊红细胞（EA）以及包被有兔IgG和鸡补体的绵羊红细胞（EAC）进行血细胞吸附，检测免疫球蛋白（Ig）G Fc和补体受体（FcR；CR）的存在情况。在脾脏中，FcR阳性细胞局限于椭球周围鞘和生发中心。在脾脏的相同区域以及法氏囊滤泡的髓质中发现了CR阳性细胞。这些淋巴区域与ABS反应强烈，但它们也被中性α-萘丁酸酯酶染色，并且在椭球周围鞘中发现了吞噬碳颗粒的细胞。此外，用环磷酰胺进行体内治疗导致明显的B淋巴细胞耗竭，这并不影响FcR活性，但显著降低了CR活性。这些数据表明，组织切片中显示的FcR活性主要局限于单核吞噬细胞，而CR阳性细胞主要是B淋巴细胞。
