一种在Percoll连续密度梯度上分离红细胞、粒细胞和单核细胞的快速单步离心法。

A rapid single centrifugation step method for the separation of erythrocytes, granulocytes and mononuclear cells on continuous density gradients of Percoll.

作者信息

Segal A W, Fortunato A, Herd T

出版信息

J Immunol Methods. 1980;32(3):209-14. doi: 10.1016/0022-1759(80)90186-6.

Abstract

A simple rapid method of separating mononuclear leucocytes, granulocytes and erythrocytes on continuous Percoll density gradients has been developed. The Percoll is mixed with increasing amounts of the blood, which forms the light phase of the gradient. Centrifugation at 400 X g for 30 min results in almost complete separation of the 3 groups of cells with high recoveries and degrees of purity. Blood samples from 3 patients with chronic granulomatous disease were separated with this technique and the pattern of cell separation was found to be different from normal.

摘要

已开发出一种在连续 Percoll 密度梯度上分离单核白细胞、粒细胞和红细胞的简单快速方法。将 Percoll 与越来越多的血液混合，形成梯度的轻相。以 400×g 离心 30 分钟可使这三组细胞几乎完全分离，回收率和纯度都很高。用该技术分离了 3 例慢性肉芽肿病患者的血样，发现细胞分离模式与正常情况不同。