Jackson S, Sogn J A, Kindt T J
J Immunol Methods. 1982;48(3):299-309. doi: 10.1016/0022-1759(82)90331-3.
Enzyme-linked immunoadsorbent assays (ELISA) specific for the allotypes of rabbit heavy chain variable region (group a) and light chain constant region (group b) have been developed. These assays utilize affinity-purified allogeneic anti-allotype antibodies to coat polyvinyl microtiter plates. After test samples are added to the plates, the same affinity-purified antibody, coupled to the enzyme peroxidase, is used to detect binding of allotype-positive IgG samples to the coated plate. These assays can detect as little as 40 ng/ml of IgG of the appropriate allotype; the assays are highly specific and allow quantitative determination of allotype-defined IgG samples. In addition, the procedure can be modified by coupling of the antibody with biotin followed by development with avidin-peroxidase; this adaptation avoids the difficulties encountered in antibody-peroxidase conjugations. The ELISA assay is not influenced by the presence of anti-allotype antibody in the test sample, giving a distinct advantage over solid-phase radioimmunoassay procedures conventionally used for the quantitative determination of rabbit allotypes.
已经开发出针对兔重链可变区（a组）和轻链恒定区（b组）同种异型的酶联免疫吸附测定（ELISA）。这些测定利用亲和纯化的同种异体抗同种异型抗体包被聚乙烯微量滴定板。将测试样品加入板中后，使用与酶过氧化物酶偶联的相同亲和纯化抗体来检测同种异型阳性IgG样品与包被板的结合。这些测定能够检测低至40 ng/ml的适当同种异型IgG；测定具有高度特异性，并且能够对同种异型定义的IgG样品进行定量测定。此外，该程序可以通过将抗体与生物素偶联，然后用抗生物素蛋白-过氧化物酶显色来进行修改；这种改进避免了在抗体-过氧化物酶偶联中遇到的困难。ELISA测定不受测试样品中抗同种异型抗体的存在的影响，这相对于传统用于兔同种异型定量测定的固相放射免疫测定程序具有明显优势。
