Chen K C, Amsel R, Eschenbach D A, Holmes K K
J Infect Dis. 1982 Mar;145(3):337-45. doi: 10.1093/infdis/145.3.337.
To assess correlations between the content of the diamines putrescine and cadaverine in vaginal fluid and the clinical manifestations of vaginitis, a rapid procedure for the determination of diamines has been developed. The procedure involves thin-layer chromatography of the dansylated vaginal washings; then the fluorescence of dansyl diamines is compared with that of dansyl alanine. For 520 college women, the result of the test for diamines was positive in 173 (88%) of 197 women with nonspecific vaginitis or vaginitis due to Trichomonas vaginalis and was negative in 291 (90%) of 323 women without nonspecific vaginitis or vaginitis due to T. vaginalis. The diamine content of vaginal fluid specimens after therapy with metronidazole was correlated with the clinical response to treatment.
为评估阴道分泌物中腐胺和尸胺含量与阴道炎临床表现之间的相关性，已开发出一种快速测定二胺的方法。该方法包括对丹磺酰化的阴道冲洗液进行薄层色谱分析；然后将丹磺酰化二胺的荧光与丹磺酰化丙氨酸的荧光进行比较。对于520名大学女性，在197名患有非特异性阴道炎或滴虫性阴道炎的女性中，173名（88%）的二胺检测结果为阳性，而在323名没有非特异性阴道炎或滴虫性阴道炎的女性中，291名（90%）的检测结果为阴性。甲硝唑治疗后阴道分泌物标本中的二胺含量与治疗的临床反应相关。
