Swain D P, Lalone B J
Am J Physiol. 1982 Apr;242(4):H713-21. doi: 10.1152/ajpheart.1982.242.4.H713.
A skeletal muscle preparation for the study of single vessel and whole organ vascular responses is presented. After surgical isolation from anesthetized rats, the gracilis muscle preparation is autoperfused via cannulation circuits. This allows the measurement of, and experimental control over, such macrovascular parameters as arterial and venous pressures and total muscle blood flow. In addition, the preparation is thin enough to allow the simultaneous study of microvessels by transilluminated microscopy. Such dual capabilities allow the preparation to be used in a wide variety of investigations and, in particular, as a primary tool in correlating microcirculatory responses with those of the whole organ in experiments designed to elucidate local control mechanisms of skeletal muscle vasculature. An example of such is demonstrated for reactive hyperemia responses simultaneously obtained at arteriolar and muscle venous effluent locations.
本文介绍了一种用于研究单血管和全器官血管反应的骨骼肌制备方法。从麻醉大鼠身上手术分离后，股薄肌制备物通过插管回路进行自体灌注。这使得能够测量并实验控制诸如动脉压和静脉压以及总肌肉血流量等大血管参数。此外，该制备物足够薄，可通过透照显微镜同时研究微血管。这种双重功能使该制备物可用于各种各样的研究，特别是作为一种主要工具，在旨在阐明骨骼肌血管系统局部控制机制的实验中，将微循环反应与全器官的反应相关联。同时在小动脉和肌肉静脉流出部位获得的反应性充血反应就是这样一个例子。
