Bernacchi A S, de Castro C R, de Ferreyra E C, de Fenos O M, Castro J A
Br J Exp Pathol. 1982 Feb;63(1):35-42.
CCl4 administration to rats caused at 3 and 6 h intense effects on the liver-cell endoplasmic reticulum such as dilatation, disorganization, detachment of ribosomes, development of extensive areas of smooth component (SER) and formation of myelin figures. 3 Amino 1,2,4 triazole administration (AT) at 3 and 6 h led to the formation of round small vesicles from the rough endoplasmic reticulum (RER), detachment of ribosomes, appearance of extensive areas of SER, appearance of elongated and distorted mitochondria with an increase in the number of peroxisomes. The administration of CCl4 to AT-pretreated animals led to a mutual cancellation of the effects on the RER, particularly remarkable at 3 h but still evident at 6 h; also, the formation of myelin figures was prevented. The other effects on cell ultrastructure exerted either by CCl4 or by AT were also observed with the combination of both chemicals. These observations reinforce the hypothesis about the need of either covalent binding of CCl4 metabolites to cellular constituents or lipid peroxidation, or both, in the origin of CCl4-induced alterations.
给大鼠注射四氯化碳在3小时和6小时时对肝细胞内质网产生强烈影响，如内质网扩张、结构紊乱、核糖体脱离、滑面内质网（SER）大片区域形成以及髓鞘样结构的形成。在3小时和6小时时注射3-氨基-1,2,4-三唑（AT）导致粗面内质网（RER）形成圆形小泡、核糖体脱离、SER大片区域出现、线粒体伸长和变形以及过氧化物酶体数量增加。给预先用AT处理的动物注射四氯化碳导致对RER的影响相互抵消，在3小时时尤为明显，但在6小时时仍很明显；此外，髓鞘样结构的形成也受到抑制。同时使用这两种化学物质时，也观察到了四氯化碳或AT对细胞超微结构产生的确其他影响。这些观察结果强化了关于在四氯化碳诱导的改变的起源中，四氯化碳代谢产物与细胞成分的共价结合或脂质过氧化作用，或两者都需要的假说。
