通过固相分析检测到的精子相关抗原抗体。

Antibodies to sperm-associated antigens detected by solid phase assays.

作者信息

Wolf D P, Rowlands D T, Haas G G

出版信息

Biol Reprod. 1982 Feb;26(1):140-6. doi: 10.1095/biolreprod26.1.140.

DOI:10.1095/biolreprod26.1.140

Abstract

Solid phase assays using radiolabeled (RIA) or enzyme-linked (ELISA) antibodies are described for the quantitation of antibodies to sperm-associated antigens. Antibodies were detected in serum from rabbits immunized with human sperm emulsified in Freund's complete adjuvant and in the plasmas or sera of vasectomized males and patients with suspected antibody-mediated infertility. The RIA and ELISA assays are comparable to each other in sensitivity and specificity, rapid in execution, and inexpensive.

摘要

描述了使用放射性标记（放射免疫分析，RIA）或酶联（酶联免疫吸附测定，ELISA）抗体的固相测定法，用于定量精子相关抗原的抗体。在用弗氏完全佐剂乳化的人精子免疫的兔子血清中，以及在输精管结扎男性和疑似抗体介导不孕症患者的血浆或血清中检测到了抗体。RIA和ELISA测定法在灵敏度和特异性方面相互可比，执行速度快且成本低。