Coombs M M
Carcinogenesis. 1982;3(2):229-30. doi: 10.1093/carcin/3.2.229.
Comparative in vitro metabolic experiments with 7,8-benzoflavone using microsomes prepared from the livers of control Sprague-Dawley rats or from rats of the same strain treated with phenobarbitone, 3-methylcholanthrene, or Aroclor 1254 (known inducers of hepatic cytochrome P450 mixed function oxidases) disclose variable amounts of the three metabolites previously reported, namely the 5,6-epoxide, 9,10-dihydro-9,10-diol, and the 5 (or 6)-phenol. Microsomes from phenobarbitone-treated rats gave in addition a small amount of the 5,6-dihydro-5,6-diol, identified by mass spectrometry and u.v. spectroscopy. The apparent discrepancy between our previous results (1) and those of Nesnow and Bergman (2) is therefore partly resolved, although the absence of the 9,10-diol from their products remains unexplained, but may have resulted from their use of rats of a different strain.
使用从对照斯普拉格-道利大鼠肝脏或用苯巴比妥、3-甲基胆蒽或多氯联苯混合物Aroclor 1254（已知的肝细胞色素P450混合功能氧化酶诱导剂）处理的同品系大鼠肝脏制备的微粒体，对7,8-苯并黄酮进行体外代谢比较实验，结果显示出先前报道的三种代谢物的含量各不相同，即5,6-环氧化物、9,10-二氢-9,10-二醇和5（或6）-苯酚。苯巴比妥处理的大鼠的微粒体还产生了少量的5,6-二氢-5,6-二醇，通过质谱和紫外光谱鉴定。因此，我们先前的结果（1）与内斯诺和伯格曼（2）的结果之间明显的差异部分得到了解决，尽管他们的产物中没有9,10-二醇的原因仍无法解释，但这可能是由于他们使用了不同品系的大鼠。
