Nathan D M, Avezzano E, Palmer J L
Clin Chem. 1982 Mar;28(3):512-5.
The irreversible formation of stable glycosylated hemoglobin proceeds through a labile intermediate that is indistinguishable, by most methods, from the stable glycosylated product. The inclusion of the labile intermediate, which changes acutely with acute blood glucose changes, detracts from the utility of the assay as an index of chronic glucose concentration. We have developed a rapid, reliable chemical method for eliminating the labile intermediate: a 30-min incubation of whole-blood samples with semi-carbazide (30 mmol/L) and aniline (12 mmol/L) at pH 5 and 38 degrees C. The semicarbazide serves as a glucose trap; the transfer of glucose from the labile glycosylated hemoglobin to the semicarbazide is catalyzed by the acidic pH and aniline. This treatment is effective in the three most commonly used assays: "high-performance" liquid chromatography, electrophoresis, and a minicolumn kit.
稳定糖化血红蛋白的不可逆形成过程中会经过一个不稳定中间体，多数方法都无法将其与稳定的糖化产物区分开来。该不稳定中间体随急性血糖变化而急剧改变，这降低了糖化血红蛋白检测作为慢性血糖浓度指标的效用。我们研发了一种快速、可靠的化学方法来消除该不稳定中间体：将全血样本在pH 5和38摄氏度条件下与氨基脲（30 mmol/L）和苯胺（12 mmol/L）孵育30分钟。氨基脲充当葡萄糖捕获剂；酸性pH值和苯胺催化葡萄糖从不稳定糖化血红蛋白转移至氨基脲。这种处理方法在三种最常用的检测方法中均有效：“高效”液相色谱法、电泳法和微柱试剂盒法。
