Yatscoff R W, Tevaarwerk G J, Clarson C L, Warnock L M
Clin Chem. 1983 Mar;29(3):543-5.
We examined the effect of fetal hemoglobin and labile glycosylated hemoglobin on a number of diverse methods used to measure glycosylated hemoglobin. Samples were supplemented with various amounts of cord blood to give proportions of fetal hemoglobin ranging from 1 to 20% of total hemoglobin concentration. Procedures in which the separation of hemoglobin A1 from the major hemoglobin A fraction is based on differences in ionic properties (cation-exchange chromatography and electrophoresis) are subject to interference by fetal hemoglobin, whereas procedures that base the quantitation on other properties (colorimetry and affinity column chromatography) are not. The same procedures that are affected by the presence of fetal hemoglobin are also subject to interference by labile glycosylated hemoglobin. We conclude that the affinity chromatographic and colorimetric methods may give a more nearly accurate determination of glycosylated hemoglobin.
我们研究了胎儿血红蛋白和不稳定糖化血红蛋白对多种用于测量糖化血红蛋白的不同方法的影响。向样本中添加不同量的脐带血，以使胎儿血红蛋白占总血红蛋白浓度的比例在1%至20%之间。基于离子性质差异（阳离子交换色谱法和电泳法）从主要血红蛋白A组分中分离血红蛋白A1的方法会受到胎儿血红蛋白的干扰，而基于其他性质（比色法和亲和柱色谱法）进行定量的方法则不受影响。受胎儿血红蛋白存在影响的相同方法也会受到不稳定糖化血红蛋白的干扰。我们得出结论，亲和色谱法和比色法可能会更准确地测定糖化血红蛋白。
