Orsulak P J, Kizuka P, Grab E, Schildkraut J J
Clin Chem. 1983 Feb;29(2):305-9.
A procedure has been developed for determining the O-methylated catecholamine metabolites, normetanephrine and metanephrine, in urine by use of radial-compression liquid chromatography followed by electrochemical detection. Normetanephrine and metanephrine are isolated from hydrolyzed urine by ion-exchange on small, commercially available, disposable columns and preconcentrated by solvent extraction. They are then separated by reversed-phase ion-pair chromatography, with use of a radial compression cartridge and radial compression module, and quantified with 3-methoxy-4-hydroxybenzylamine as internal standard. Normetanephrine, metanephrine, and the internal standard are separated from interfering peaks in about 15 min. The method is applicable to the relatively low amounts of normetanephrine (100-600 micrograms/24 h) and metanephrine (50-400 micrograms/24 h) found in normal subjects and patients with depressive disorders or hypertension. Within-day CVs ranged from 1.1 to 2.2% for normetanephrine and 1.2 to 6.9% for metanephrine; the corresponding between-day CVs were 4.9 and 5.7% over these ranges.
已开发出一种程序，通过径向压缩液相色谱法随后进行电化学检测来测定尿液中的O-甲基化儿茶酚胺代谢物、去甲变肾上腺素和变肾上腺素。去甲变肾上腺素和变肾上腺素通过在市售的一次性小柱上进行离子交换从水解尿液中分离出来，并通过溶剂萃取进行预浓缩。然后使用径向压缩柱套和径向压缩模块通过反相离子对色谱法进行分离，并以3-甲氧基-4-羟基苄胺作为内标进行定量。去甲变肾上腺素、变肾上腺素和内标在约15分钟内与干扰峰分离。该方法适用于正常受试者以及患有抑郁症或高血压的患者体内相对少量的去甲变肾上腺素（100 - 600微克/24小时）和变肾上腺素（50 - 400微克/24小时）。去甲变肾上腺素的日内变异系数范围为1.1%至2.2%，变肾上腺素为1.2%至6.9%；在这些范围内相应的日间变异系数分别为4.9%和5.7%。
